Stable transformation of plants

Huw D Jones, Caroline A. Sparks

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Citations (SciVal)

Abstract

This chapter provides an overview of the main steps in the process to produce stably transformed plants. Most transformation methods use tissue culture to recover adult plants from regenerable explants and can be divided into three stages: (1) choice and preparation of explant tissue, (2) deoxyribonucleic acid (DNA) delivery, (3) callus induction/regeneration and selection. Each of these stages is introduced from a general perspective and a detailed protocol for our exemplar species, wheat, is given. We focus here on DNA delivery by particle bombardment as Agrobacterium-mediated transformation methods for wheat are reported elsewhere.

Original languageEnglish
Title of host publicationPlant Genomics
Subtitle of host publicationMethods and Protocols
EditorsJ. P. Gustafson, Peter Langridge, Daryl J. Somers
PublisherSpringer Nature
Pages111-130
Number of pages20
Volume513
ISBN (Electronic)978-1-59745-427-8
ISBN (Print)978-1-58829-997-0
DOIs
Publication statusPublished - 2009

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume513
ISSN (Electronic)1064-3745

Keywords

  • Biolistics
  • DNA, Recombinant
  • Genetic Techniques
  • Plants, Genetically Modified
  • Tissue Culture Techniques
  • Transformation, Genetic
  • Triticum

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