Synthetic peptide segments from Escherichia coli porin OmpF constitute leukocyte activators

H. M. Vordermeier; P. Hoffmann; F. O. Gombert; G. Jung; W. G. Bessler

doi:10.1128/iai.58.8.2719-2724.1990

Synthetic peptide segments from Escherichia coli porin OmpF constitute leukocyte activators

H. M. Vordermeier, P. Hoffmann, F. O. Gombert, G. Jung, W. G. Bessler^*

^*Corresponding author for this work

Bovine TB, diseases and control

University of Freiburg

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

After characterization of the porin OmpF and selection of molecular structures responsible for leukocyte activation by using computer-assisted epitope analysis, the analogs OmpF (153-174) (containing amino acids 153 to 174), OmpF (157-174), and OmpF (275-284) were synthesized and tested. Like the native protein, the segments were mitogenic for BALB/c splenocytes and induced B lymphocyte differentiation into antibody-producing plasma cells and tumor cytotoxicity of macrophages against the fibroblast cell line L929. We thus demonstrated that defined peptide segments are responsible for the leukocyte-activating properties of a major bacterial surface protein.

Original language	English
Pages (from-to)	2719-2724
Number of pages	6
Journal	Infection and Immunity
Volume	58
Issue number	8
DOIs	https://doi.org/10.1128/iai.58.8.2719-2724.1990
Publication status	Published - 01 Aug 1990

Keywords

Animals
B-Lymphocytes/immunology
Bacterial Outer Membrane Proteins/chemical synthesis
Bone Marrow/immunology
Epitopes
Escherichia coli/immunology
Lymphocyte Activation/immunology
Macrophages/immunology
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Peptides/chemical synthesis
Porins
Spleen/immunology
Tumor Cells, Cultured

UN SDGs

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10.1128/iai.58.8.2719-2724.1990

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title = "Synthetic peptide segments from Escherichia coli porin OmpF constitute leukocyte activators",

abstract = "After characterization of the porin OmpF and selection of molecular structures responsible for leukocyte activation by using computer-assisted epitope analysis, the analogs OmpF (153-174) (containing amino acids 153 to 174), OmpF (157-174), and OmpF (275-284) were synthesized and tested. Like the native protein, the segments were mitogenic for BALB/c splenocytes and induced B lymphocyte differentiation into antibody-producing plasma cells and tumor cytotoxicity of macrophages against the fibroblast cell line L929. We thus demonstrated that defined peptide segments are responsible for the leukocyte-activating properties of a major bacterial surface protein.",

keywords = "Animals, B-Lymphocytes/immunology, Bacterial Outer Membrane Proteins/chemical synthesis, Bone Marrow/immunology, Epitopes, Escherichia coli/immunology, Lymphocyte Activation/immunology, Macrophages/immunology, Mice, Mice, Inbred BALB C, Mice, Inbred C3H, Peptides/chemical synthesis, Porins, Spleen/immunology, Tumor Cells, Cultured",

author = "Vordermeier, {H. M.} and P. Hoffmann and Gombert, {F. O.} and G. Jung and Bessler, {W. G.}",

year = "1990",

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doi = "10.1128/iai.58.8.2719-2724.1990",

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TY - JOUR

T1 - Synthetic peptide segments from Escherichia coli porin OmpF constitute leukocyte activators

AU - Vordermeier, H. M.

AU - Hoffmann, P.

AU - Gombert, F. O.

AU - Jung, G.

AU - Bessler, W. G.

PY - 1990/8/1

Y1 - 1990/8/1

N2 - After characterization of the porin OmpF and selection of molecular structures responsible for leukocyte activation by using computer-assisted epitope analysis, the analogs OmpF (153-174) (containing amino acids 153 to 174), OmpF (157-174), and OmpF (275-284) were synthesized and tested. Like the native protein, the segments were mitogenic for BALB/c splenocytes and induced B lymphocyte differentiation into antibody-producing plasma cells and tumor cytotoxicity of macrophages against the fibroblast cell line L929. We thus demonstrated that defined peptide segments are responsible for the leukocyte-activating properties of a major bacterial surface protein.

AB - After characterization of the porin OmpF and selection of molecular structures responsible for leukocyte activation by using computer-assisted epitope analysis, the analogs OmpF (153-174) (containing amino acids 153 to 174), OmpF (157-174), and OmpF (275-284) were synthesized and tested. Like the native protein, the segments were mitogenic for BALB/c splenocytes and induced B lymphocyte differentiation into antibody-producing plasma cells and tumor cytotoxicity of macrophages against the fibroblast cell line L929. We thus demonstrated that defined peptide segments are responsible for the leukocyte-activating properties of a major bacterial surface protein.

KW - Animals

KW - B-Lymphocytes/immunology

KW - Bacterial Outer Membrane Proteins/chemical synthesis

KW - Bone Marrow/immunology

KW - Epitopes

KW - Escherichia coli/immunology

KW - Lymphocyte Activation/immunology

KW - Macrophages/immunology

KW - Mice

KW - Mice, Inbred BALB C

KW - Mice, Inbred C3H

KW - Peptides/chemical synthesis

KW - Porins

KW - Spleen/immunology

KW - Tumor Cells, Cultured

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U2 - 10.1128/iai.58.8.2719-2724.1990

DO - 10.1128/iai.58.8.2719-2724.1990

M3 - Article

C2 - 1695209

AN - SCOPUS:0025291333

SN - 0019-9567

VL - 58

SP - 2719

EP - 2724

JO - Infection and Immunity

JF - Infection and Immunity

IS - 8

ER -

Synthetic peptide segments from Escherichia coli porin OmpF constitute leukocyte activators

Abstract

Keywords

UN SDGs

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