Synthetic peptide vaccination in cattle: Induction of strong cellular immune responses against peptides derived from the Mycobacterium bovis antigen Rv3019c

H. Martin Vordermeier*, Reno Pontarollo, Brenda Karvonen, Paul Cockle, Rolf Hecker, Mahavir Singh, Lorne A. Babiuk, R. Glyn Hewinson, Sylvia Van Drunen Littel-Van Den Hurk

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Citations (SciVal)

Abstract

Fully synthetic peptide vaccines possess attractive cost and safety attributes. However, peptide vaccines that induce cell-mediated immunity require both selection of appropriate peptides and the development of adjuvant formulations supporting the induction of cellular immunity. An adjuvant formulation composed of emulsigen and the synthetic CpG motif containing ODN2007 was tested in cattle for its ability to induce cellular immunity after peptide vaccination, and compared to Rv3019c DNA vaccination. Peptides from the protective Mycobacterium bovis antigen Rv3019c were included into the vaccine on the basis of their frequent and strong recognition by T cells from M. bovis infected or BCG vaccinated cattle. Following peptide vaccination, strong IFN-γ and proliferative T cell responses were observed. Proliferative, but no significant IFN-γ responses were induced by DNA vaccination. Peptide vaccination boosted responses primed by DNA vaccination. In conclusion, emulsigen and CpG motif containing ODN constitute a promising adjuvant formulation to deliver peptides to veterinary species.

Original languageEnglish
Pages (from-to)4375-4384
Number of pages10
JournalVaccine
Volume23
Issue number35
Early online date10 May 2005
DOIs
Publication statusPublished - 15 Aug 2005

Keywords

  • Adjuvant
  • Bovine tuberculosis
  • Cattle
  • Peptides
  • Vaccine

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