T-DNA mutagenesis in Brachypodium distachyon

Vera Thole, Antoine Peraldi, Barbara Worland, Paul Nicholson, John H. Doonan, Philippe Vain

Research output: Contribution to journalArticlepeer-review

45 Citations (SciVal)

Abstract

During the past decade, Brachypodium distachyon has emerged as an attractive experimental system and genomics model for grass research. Numerous molecular tools and genomics resources have already been developed. Functional genomics resources, including mutant collections, expression/tiling microarray, mapping populations, and genome re-sequencing for natural accessions, are rapidly being developed and made available to the community. In this article, the focus is on the current status of systematic T-DNA mutagenesis in Brachypodium. Large collections of T-DNA-tagged lines are being generated by a community of laboratories in the context of the International Brachypodium Tagging Consortium. To date, >13 000 lines produced by the BrachyTAG programme and USDA-ARS Western Regional Research Center are available by online request. The utility of these mutant collections is illustrated with some examples from the BrachyTAG collection at the John Innes Centre—such as those in the eukaryotic initiation factor 4A (eIF4A) and brassinosteroid insensitive-1 (BRI1) genes. A series of other mutants exhibiting growth phenotypes is also presented. These examples highlight the value of Brachypodium as a model for grass functional genomics.
Original languageEnglish
Pages (from-to)567-576
Number of pages10
JournalJournal of Experimental Botany
Volume63
Issue number2
DOIs
Publication statusPublished - 16 Nov 2011

Keywords

  • brachypodium
  • BRI1
  • DEV mutant
  • eIF4A
  • functional genomics
  • grass
  • IBTC
  • model
  • mutagenesis
  • T-DNA mutant

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