Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses

Marcia M. de O. Buanafina; Tim Langdon; H. Hicks; Barbara D. Hauck; Sue J. Dalton; Phillip Morris

Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses

Marcia M. de O. Buanafina, Tim Langdon, H. Hicks, Barbara D. Hauck, Sue J. Dalton, Phillip Morris

Department of Life Sciences

Research output: Chapter in Book/Report/Conference proceeding › Conference Proceeding (Non-Journal item)

Abstract

Transgenic plants of Festuca arundinacea and Lolium multiflorum were produced expressing a ferulic acid esterase (FAE) gene isolated from Aspergillus niger, following microprojectile bombardment of cell cultures. The level of FAE activity measured from different vectors targeting FAE to the vacuole, ER and apoplast under constitutive promoters showed that the activity was highest in young leaves and increased along the leaf lamina. FAE expression resulted in release of monomeric and dimeric ferulic acids from cell walls on cell death and this was enhanced several fold by the addition of recombinant xylanase. The effect of FAE expression on the monomeric and dimeric cell wall ester linked ferulate content in comparison to control (non-transformed) plants is reported.

Original language	English
Title of host publication	Multifunctional Grassland: Quality Forages, Animal Products and Landscapes
Editors	J- L. Durand, J-C. Emile, C. Huyghe, G. Lemaire
Publisher	British Grassland Society
Pages	66-69
Number of pages	4
Volume	7
ISBN (Print)	2950411037
Publication status	Published - 2002
Event	Proceedings of the 19th General Meeting of the European Grassland Federation (EGF) - La Rochelle, France Duration: 27 May 2002 → 30 May 2002

Publication series

Name	Grassland Science in Europe

Conference

Conference	Proceedings of the 19th General Meeting of the European Grassland Federation (EGF)
Country/Territory	France
City	La Rochelle
Period	27 May 2002 → 30 May 2002

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Buanafina, M. M. D. O., Langdon, T., Hicks, H., Hauck, B. D., Dalton, S. J., & Morris, P. (2002). Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses. In J. L. Durand, J.-C. Emile, C. Huyghe, & G. Lemaire (Eds.), Multifunctional Grassland: Quality Forages, Animal Products and Landscapes (Vol. 7, pp. 66-69). (Grassland Science in Europe). British Grassland Society. http://hdl.handle.net/2160/3802

Buanafina, Marcia M. de O. ; Langdon, Tim ; Hicks, H. et al. / Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses. Multifunctional Grassland: Quality Forages, Animal Products and Landscapes. editor / J- L. Durand ; J-C. Emile ; C. Huyghe ; G. Lemaire. Vol. 7 British Grassland Society, 2002. pp. 66-69 (Grassland Science in Europe).

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title = "Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses",

abstract = "Transgenic plants of Festuca arundinacea and Lolium multiflorum were produced expressing a ferulic acid esterase (FAE) gene isolated from Aspergillus niger, following microprojectile bombardment of cell cultures. The level of FAE activity measured from different vectors targeting FAE to the vacuole, ER and apoplast under constitutive promoters showed that the activity was highest in young leaves and increased along the leaf lamina. FAE expression resulted in release of monomeric and dimeric ferulic acids from cell walls on cell death and this was enhanced several fold by the addition of recombinant xylanase. The effect of FAE expression on the monomeric and dimeric cell wall ester linked ferulate content in comparison to control (non-transformed) plants is reported.",

author = "Buanafina, {Marcia M. de O.} and Tim Langdon and H. Hicks and Hauck, {Barbara D.} and Dalton, {Sue J.} and Phillip Morris",

note = "Buanafina, M. M. de O., Langdon, T., Hicks, H., Hauck, B., Dalton, S. J., Morris, P. (2002). Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses. Pages 66-69 in: , Durand, J-L., Emile, J-C., Huyghe, C., Lemaire, G. (Eds). Multifunctional Grassland: Quality Forages, Animal Products and Landscapes. Grassland Science in Europe 7, ISBN:2950411037. Proceedings of the 19th General Meeting of the European Grassland Federation, La Rochelle, France, 27-30 May 2002. RONO: 1700 6729; 1700 3120; Proceedings of the 19th General Meeting of the European Grassland Federation (EGF) ; Conference date: 27-05-2002 Through 30-05-2002",

year = "2002",

language = "English",

isbn = "2950411037",

volume = "7",

series = "Grassland Science in Europe",

publisher = "British Grassland Society",

pages = "66--69",

editor = "Durand, {J- L.} and J-C. Emile and C. Huyghe and G. Lemaire",

booktitle = "Multifunctional Grassland: Quality Forages, Animal Products and Landscapes",

}

Buanafina, MMDO, Langdon, T, Hicks, H, Hauck, BD, Dalton, SJ & Morris, P 2002, Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses. in JL Durand, J-C Emile, C Huyghe & G Lemaire (eds), Multifunctional Grassland: Quality Forages, Animal Products and Landscapes. vol. 7, Grassland Science in Europe, British Grassland Society, pp. 66-69, Proceedings of the 19th General Meeting of the European Grassland Federation (EGF), La Rochelle, France, 27 May 2002. <http://hdl.handle.net/2160/3802>

Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses. / Buanafina, Marcia M. de O.; Langdon, Tim; Hicks, H. et al.
Multifunctional Grassland: Quality Forages, Animal Products and Landscapes. ed. / J- L. Durand; J-C. Emile; C. Huyghe; G. Lemaire. Vol. 7 British Grassland Society, 2002. p. 66-69 (Grassland Science in Europe).

Research output: Chapter in Book/Report/Conference proceeding › Conference Proceeding (Non-Journal item)

TY - GEN

T1 - Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses

AU - Buanafina, Marcia M. de O.

AU - Langdon, Tim

AU - Hicks, H.

AU - Hauck, Barbara D.

AU - Dalton, Sue J.

AU - Morris, Phillip

N1 - Buanafina, M. M. de O., Langdon, T., Hicks, H., Hauck, B., Dalton, S. J., Morris, P. (2002). Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses. Pages 66-69 in: , Durand, J-L., Emile, J-C., Huyghe, C., Lemaire, G. (Eds). Multifunctional Grassland: Quality Forages, Animal Products and Landscapes. Grassland Science in Europe 7, ISBN:2950411037. Proceedings of the 19th General Meeting of the European Grassland Federation, La Rochelle, France, 27-30 May 2002. RONO: 1700 6729; 1700 3120

PY - 2002

Y1 - 2002

N2 - Transgenic plants of Festuca arundinacea and Lolium multiflorum were produced expressing a ferulic acid esterase (FAE) gene isolated from Aspergillus niger, following microprojectile bombardment of cell cultures. The level of FAE activity measured from different vectors targeting FAE to the vacuole, ER and apoplast under constitutive promoters showed that the activity was highest in young leaves and increased along the leaf lamina. FAE expression resulted in release of monomeric and dimeric ferulic acids from cell walls on cell death and this was enhanced several fold by the addition of recombinant xylanase. The effect of FAE expression on the monomeric and dimeric cell wall ester linked ferulate content in comparison to control (non-transformed) plants is reported.

AB - Transgenic plants of Festuca arundinacea and Lolium multiflorum were produced expressing a ferulic acid esterase (FAE) gene isolated from Aspergillus niger, following microprojectile bombardment of cell cultures. The level of FAE activity measured from different vectors targeting FAE to the vacuole, ER and apoplast under constitutive promoters showed that the activity was highest in young leaves and increased along the leaf lamina. FAE expression resulted in release of monomeric and dimeric ferulic acids from cell walls on cell death and this was enhanced several fold by the addition of recombinant xylanase. The effect of FAE expression on the monomeric and dimeric cell wall ester linked ferulate content in comparison to control (non-transformed) plants is reported.

M3 - Conference Proceeding (Non-Journal item)

SN - 2950411037

VL - 7

T3 - Grassland Science in Europe

SP - 66

EP - 69

BT - Multifunctional Grassland: Quality Forages, Animal Products and Landscapes

A2 - Durand, J- L.

A2 - Emile, J-C.

A2 - Huyghe, C.

A2 - Lemaire, G.

PB - British Grassland Society

T2 - Proceedings of the 19th General Meeting of the European Grassland Federation (EGF)

Y2 - 27 May 2002 through 30 May 2002

ER -

Buanafina MMDO, Langdon T, Hicks H, Hauck BD, Dalton SJ, Morris P. Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses. In Durand JL, Emile JC, Huyghe C, Lemaire G, editors, Multifunctional Grassland: Quality Forages, Animal Products and Landscapes. Vol. 7. British Grassland Society. 2002. p. 66-69. (Grassland Science in Europe).

Targeted expression of a ferulic acid esterase from Aspergillus niger in leaves of forage grasses

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