The 22 kDa component of the protein complex on the surface of Plasmodium falciparum merozoites is derived from a larger precursor, merozoite surface protein 7

Justin A. Pachebat, Irene T. Ling, Munira Grainger, Carlotta Trucco, Steven Howell, Delmiro Fernandez-Reyes, Ruwani Gunaratne, Anthony A. Holder

Research output: Contribution to journalArticlepeer-review

96 Citations (SciVal)

Abstract

The gene coding for merozoite surface protein 7 has been identified and sequenced in three lines of Plasmodium falciparum. The gene encodes a 351 amino acid polypeptide that is the precursor of a 22-kDa protein (MSP7(22)) on the merozoite surface and non-covalently associated with merozoite surface protein 1 (MSP1) complex shed from the surface at erythrocyte invasion. A second 19-kDa component of the complex (MSP7(19)) was shown to be derived from MSP7(22) and the complete primary structure of this polypeptide was confirmed by mass spectrometry. The protein sequence contains several predicted helical and two beta elements, but has no similarity with sequences outside the Plasmodium databases. Four sites of sequence variation were identified in MSP7, all within the MSP7(22) region. The MSP7 gene is expressed in mature schizonts, at the same time as other merozoite surface protein genes. It is proposed that MSP7(22) is the result of cleavage by a protease that may also cleave MSP1 and MSP6. A related gene was identified and cloned from the rodent malaria parasite, Plasmodium yoelii YM; at the amino acid level this sequence was 23% identical and 50% similar to that of P. falciparum MSP7.

Original languageEnglish
Pages (from-to)83-9
Number of pages7
JournalMolecular and Biochemical Parasitology
Volume117
Issue number1
Early online date04 Sept 2001
DOIs
Publication statusPublished - 28 Sept 2001

Keywords

  • Amino Acid Sequence
  • Animals
  • Conserved Sequence
  • Membrane Proteins
  • Merozoite Surface Protein 1
  • Molecular Sequence Data
  • Plasmodium falciparum
  • Protein Precursors
  • Protozoan Proteins
  • Sequence Analysis, DNA

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