Abstract
Retrotransposons are a major agent of genome evolution. Various molecular marker systems have been
developed that exploit the ubiquitous nature of these genetic elements and their property of stable integration
into dispersed chromosomal loci that are polymorphic within species. The key methods, SSAP, IRAP,
REMAP, RBIP, and ISBP, all detect the sites at which the retrotransposon DNA, which is conserved
between families of elements, is integrated into the genome. Marker systems exploiting these methods can
be easily developed and inexpensively deployed in the absence of extensive genome sequence data. They
offer access to the dynamic and polymorphic, nongenic portion of the genome and thereby complement
methods, such as gene-derived SNPs, that target primarily the genic fraction.
developed that exploit the ubiquitous nature of these genetic elements and their property of stable integration
into dispersed chromosomal loci that are polymorphic within species. The key methods, SSAP, IRAP,
REMAP, RBIP, and ISBP, all detect the sites at which the retrotransposon DNA, which is conserved
between families of elements, is integrated into the genome. Marker systems exploiting these methods can
be easily developed and inexpensively deployed in the absence of extensive genome sequence data. They
offer access to the dynamic and polymorphic, nongenic portion of the genome and thereby complement
methods, such as gene-derived SNPs, that target primarily the genic fraction.
| Original language | English |
|---|---|
| Publisher | Springer Nature |
| Number of pages | 39 |
| Volume | 859 |
| Edition | Methods in Molecular Biology |
| DOIs | |
| Publication status | Published - 2012 |
Publication series
| Name | Methods in Molecular Biology, Mobile Genetic Elements: Protocols and Genomic Applications, Methods in Molecular Biology, Yves Bigot (ed.) |
|---|---|
| Publisher | Springer Science+Business Media |
| Volume | 859 |