The cap-binding complex modulates ABA-responsive transcript splicing during germination in barley (Hordeum vulgare)

Ewa Sybilska; Anna Collin; Bahareh Sadat Haddadi; Luis A.J. Mur; Manfred Beckmann; Wenbin Guo; Craig G. Simpson; Agata Daszkowska-Golec

doi:10.1038/s41598-024-69373-9

The cap-binding complex modulates ABA-responsive transcript splicing during germination in barley (Hordeum vulgare)

Ewa Sybilska, Anna Collin, Bahareh Sadat Haddadi, Luis A.J. Mur, Manfred Beckmann, Wenbin Guo, Craig G. Simpson, Agata Daszkowska-Golec^*

^*Corresponding author for this work

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

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Abstract

To decipher the molecular bases governing seed germination, this study presents the pivotal role of the cap-binding complex (CBC), comprising CBP20 and CBP80, in modulating the inhibitory effects of abscisic acid (ABA) in barley. Using both single and double barley mutants in genes encoding the CBC, we revealed that the double mutant hvcbp20.ab/hvcbp80.b displays ABA insensitivity, in stark contrast to the hypersensitivity observed in single mutants during germination. Our comprehensive transcriptome and metabolome analysis not only identified significant alterations in gene expression and splicing patterns but also underscored the regulatory nexus among CBC, ABA, and brassinosteroid (BR) signaling pathways.

Original language	English
Article number	18278
Journal	Scientific Reports
Volume	14
Issue number	1
Early online date	07 Aug 2024
DOIs	https://doi.org/10.1038/s41598-024-69373-9
Publication status	E-pub ahead of print - 07 Aug 2024

Keywords

Transcriptome
Cap-binding complex
ABA
Germination
Embryo
Barley
Alternative splicing
Seeds
Hordeum
Signal Transduction
Abscisic Acid
Gene Expression Profiling
RNA Splicing
Gene Expression Regulation, Plant
Plant Proteins
Mutation
RNA Cap-Binding Proteins
Cap-binding Complex
RNA Cap-Binding Proteins/metabolism
Germination/genetics
Abscisic Acid/metabolism
Seeds/growth & development
Hordeum/genetics
Plant Proteins/genetics

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41598_2024_Article_69373.pdfFinal published version, 3.95 MBLicence: CC BY

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title = "The cap-binding complex modulates ABA-responsive transcript splicing during germination in barley (Hordeum vulgare)",

abstract = "To decipher the molecular bases governing seed germination, this study presents the pivotal role of the cap-binding complex (CBC), comprising CBP20 and CBP80, in modulating the inhibitory effects of abscisic acid (ABA) in barley. Using both single and double barley mutants in genes encoding the CBC, we revealed that the double mutant hvcbp20.ab/hvcbp80.b displays ABA insensitivity, in stark contrast to the hypersensitivity observed in single mutants during germination. Our comprehensive transcriptome and metabolome analysis not only identified significant alterations in gene expression and splicing patterns but also underscored the regulatory nexus among CBC, ABA, and brassinosteroid (BR) signaling pathways.",

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AU - Sadat Haddadi, Bahareh

AU - Mur, Luis A.J.

AU - Beckmann, Manfred

AU - Guo, Wenbin

AU - Simpson, Craig G.

AU - Daszkowska-Golec, Agata

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N2 - To decipher the molecular bases governing seed germination, this study presents the pivotal role of the cap-binding complex (CBC), comprising CBP20 and CBP80, in modulating the inhibitory effects of abscisic acid (ABA) in barley. Using both single and double barley mutants in genes encoding the CBC, we revealed that the double mutant hvcbp20.ab/hvcbp80.b displays ABA insensitivity, in stark contrast to the hypersensitivity observed in single mutants during germination. Our comprehensive transcriptome and metabolome analysis not only identified significant alterations in gene expression and splicing patterns but also underscored the regulatory nexus among CBC, ABA, and brassinosteroid (BR) signaling pathways.

AB - To decipher the molecular bases governing seed germination, this study presents the pivotal role of the cap-binding complex (CBC), comprising CBP20 and CBP80, in modulating the inhibitory effects of abscisic acid (ABA) in barley. Using both single and double barley mutants in genes encoding the CBC, we revealed that the double mutant hvcbp20.ab/hvcbp80.b displays ABA insensitivity, in stark contrast to the hypersensitivity observed in single mutants during germination. Our comprehensive transcriptome and metabolome analysis not only identified significant alterations in gene expression and splicing patterns but also underscored the regulatory nexus among CBC, ABA, and brassinosteroid (BR) signaling pathways.

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The cap-binding complex modulates ABA-responsive transcript splicing during germination in barley (Hordeum vulgare)

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