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The ability to adapt growth and development to temperature variations is crucial to generate plant varieties resilient to predicted temperature changes. However, the mechanisms underlying plant response to progressive increases in temperature have just started to be elucidated. Here, we report that the Cyclin‐dependent Kinase G1 (CDKG1) is a central element in a thermo‐sensitive mRNA splicing cascade that transduces changes in ambient temperature into differential expression of the fundamental spliceosome component, ATU2AF65A. CDKG1 is alternatively spliced in a temperature‐dependent manner. We found that this process is partly dependent on both the Cyclin‐dependent Kinase G2 (CDKG2) and the interacting co‐factor CYCLIN L1 resulting in two distinct messenger RNAs. Relative abundance of both CDKG1 transcripts correlates with ambient temperature and possibly with different expression levels of the associated protein isoforms. Both CDKG1 alternative transcripts are necessary to fully complement the expression of ATU2AF65A across the temperature range. Our data support a previously unidentified temperature‐dependent mechanism based on the alternative splicing of CDKG1 and regulated by CDKG2 and CYCLIN L1. We propose that changes in ambient temperature affect the relative abundance of CDKG1 transcripts and this in turn translates into differential CDKG1 protein expression coordinating the alternative splicing of ATU2AF65A.
|Number of pages||13|
|Early online date||30 Mar 2018|
|Publication status||Published - 30 Jun 2018|
- alternative splicing
- cyclin-dependent kinases
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- Faculty of Earth and Life Sciences, Institute of Biological, Environmental & Rural Sciences (IBERS) - Chair (DLM)
Person: Teaching And Research
- 1 Finished
Can cyclin dependant kinase activity be manipulated to control chromosome pairing and recombination in plants?
Doonan, J., Fernandez Fuentes, N. & Jenkins, G.
Biotechnology and Biological Sciences Research Council
01 Jan 2015 → 31 Aug 2018
Project: Externally funded research