The cytochrome p450 complement (CYPome) of Streptomyces coelicolor A3(2)

Steven Lewis Kelly; M. R. Waterman; T. Skaug; Colin J. Jackson; Diane Elizabeth Kelly; L. M. Podust; Douglas B. Kell; H. L. Song; David Christopher Lamb

doi:10.1074/jbc.M111109200

The cytochrome p450 complement (CYPome) of Streptomyces coelicolor A3(2)

Steven Lewis Kelly, M. R. Waterman, T. Skaug, Colin J. Jackson, Diane Elizabeth Kelly, L. M. Podust, Douglas B. Kell, H. L. Song, David Christopher Lamb

Department of Life Sciences

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126 Citations (Scopus)

Abstract

In the present study we describe the complete cytochrome P450 complement, the “CYPome,” of Streptomyces coelicolor A3(2). Eighteen cytochromes P450 (CYP) are described, in contrast to the absence of CYPs in Escherichia coli, and the twenty observed in Mycobacterium tuberculosis. Here we confirm protein identity as cytochromes P450 by heterologous expression in E. coli and measurement of reduced carbon monoxide difference spectra. We also report on their arrangement in the linear chromosome and relatedness to other CYPs in the superfamily. The future development of manipulation of antibiotic pathways and the use of streptomycetes in bioremediation and biotransformations will involve many of the new CYP forms identified here.

Original language	English
Pages (from-to)	24000-24005
Number of pages	6
Journal	Journal of Biological Chemistry
Volume	277
DOIs	https://doi.org/10.1074/jbc.M111109200
Publication status	Published - 09 Apr 2002

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title = "The cytochrome p450 complement (CYPome) of Streptomyces coelicolor A3(2)",

abstract = "In the present study we describe the complete cytochrome P450 complement, the “CYPome,” of Streptomyces coelicolor A3(2). Eighteen cytochromes P450 (CYP) are described, in contrast to the absence of CYPs in Escherichia coli, and the twenty observed in Mycobacterium tuberculosis. Here we confirm protein identity as cytochromes P450 by heterologous expression in E. coli and measurement of reduced carbon monoxide difference spectra. We also report on their arrangement in the linear chromosome and relatedness to other CYPs in the superfamily. The future development of manipulation of antibiotic pathways and the use of streptomycetes in bioremediation and biotransformations will involve many of the new CYP forms identified here.",

author = "Kelly, {Steven Lewis} and Waterman, {M. R.} and T. Skaug and Jackson, {Colin J.} and Kelly, {Diane Elizabeth} and Podust, {L. M.} and Kell, {Douglas B.} and Song, {H. L.} and Lamb, {David Christopher}",

note = "Lamb, D. C., Skaug, T., Song, H. L., Jackson, C. J., Podust, L. M., Waterman, M. R., Kell, D. B., Kelly, D. E., Kelly, S. L. (2002). The cytochrome p450 complement (CYPome) of Streptomyces coelicolor A3(2). Journal of Biological Chemistry, 277, 24000-24005. Sponsorship: BBSRC/MRC",

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doi = "10.1074/jbc.M111109200",

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T1 - The cytochrome p450 complement (CYPome) of Streptomyces coelicolor A3(2)

AU - Kelly, Steven Lewis

AU - Waterman, M. R.

AU - Skaug, T.

AU - Jackson, Colin J.

AU - Kelly, Diane Elizabeth

AU - Podust, L. M.

AU - Kell, Douglas B.

AU - Song, H. L.

AU - Lamb, David Christopher

N1 - Lamb, D. C., Skaug, T., Song, H. L., Jackson, C. J., Podust, L. M., Waterman, M. R., Kell, D. B., Kelly, D. E., Kelly, S. L. (2002). The cytochrome p450 complement (CYPome) of Streptomyces coelicolor A3(2). Journal of Biological Chemistry, 277, 24000-24005. Sponsorship: BBSRC/MRC

PY - 2002/4/9

Y1 - 2002/4/9

N2 - In the present study we describe the complete cytochrome P450 complement, the “CYPome,” of Streptomyces coelicolor A3(2). Eighteen cytochromes P450 (CYP) are described, in contrast to the absence of CYPs in Escherichia coli, and the twenty observed in Mycobacterium tuberculosis. Here we confirm protein identity as cytochromes P450 by heterologous expression in E. coli and measurement of reduced carbon monoxide difference spectra. We also report on their arrangement in the linear chromosome and relatedness to other CYPs in the superfamily. The future development of manipulation of antibiotic pathways and the use of streptomycetes in bioremediation and biotransformations will involve many of the new CYP forms identified here.

AB - In the present study we describe the complete cytochrome P450 complement, the “CYPome,” of Streptomyces coelicolor A3(2). Eighteen cytochromes P450 (CYP) are described, in contrast to the absence of CYPs in Escherichia coli, and the twenty observed in Mycobacterium tuberculosis. Here we confirm protein identity as cytochromes P450 by heterologous expression in E. coli and measurement of reduced carbon monoxide difference spectra. We also report on their arrangement in the linear chromosome and relatedness to other CYPs in the superfamily. The future development of manipulation of antibiotic pathways and the use of streptomycetes in bioremediation and biotransformations will involve many of the new CYP forms identified here.

U2 - 10.1074/jbc.M111109200

DO - 10.1074/jbc.M111109200

M3 - Article

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VL - 277

SP - 24000

EP - 24005

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

ER -

The cytochrome p450 complement (CYPome) of Streptomyces coelicolor A3(2)

Abstract

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