The cytochrome p450 complement (CYPome) of Streptomyces coelicolor A3(2)

Steven Lewis Kelly, M. R. Waterman, T. Skaug, Colin J. Jackson, Diane Elizabeth Kelly, L. M. Podust, Douglas B. Kell, H. L. Song, David Christopher Lamb

Research output: Contribution to journalArticlepeer-review

121 Citations (SciVal)

Abstract

In the present study we describe the complete cytochrome P450 complement, the “CYPome,” of Streptomyces coelicolor A3(2). Eighteen cytochromes P450 (CYP) are described, in contrast to the absence of CYPs in Escherichia coli, and the twenty observed in Mycobacterium tuberculosis. Here we confirm protein identity as cytochromes P450 by heterologous expression in E. coli and measurement of reduced carbon monoxide difference spectra. We also report on their arrangement in the linear chromosome and relatedness to other CYPs in the superfamily. The future development of manipulation of antibiotic pathways and the use of streptomycetes in bioremediation and biotransformations will involve many of the new CYP forms identified here.
Original languageEnglish
Pages (from-to)24000-24005
Number of pages6
JournalJournal of Biological Chemistry
Volume277
DOIs
Publication statusPublished - 09 Apr 2002

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