TY - JOUR
T1 - The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees
AU - Allainguillaume, Joel
AU - Quainoo, A. K.
AU - Wetten, A. C.
N1 - Quainoo, A. K., Wetten, A. C., Allainguillaume, J. (2008). The effectiveness of somatic embryogenesis in eliminating the cocoa swollen shoot virus from infected cocoa trees. Journal of Virological Methods, 149, (1), 91-96.
IMPF: 02.08 RONO: 00
PY - 2008/4/1
Y1 - 2008/4/1
N2 - Investigations were undertaken on the use of somatic embryogenesis to generate cocoa swollen shoot virus (CSSV) disease free clonal propagules from infected trees. Polymerase chain reaction (PCR) capillary electrophoresis revealed the presence of CSSV in all the callus tissues induced from the CSSV-infected Amelonado cocoa trees (T1, T2 and T4). The virus was transmitted to primary somatic embryos induced from the infected callus tissues at the rate of 10 (19%), 18 (14%) and 16 (15%) for T1, T2 and T4, respectively. Virus free primary somatic embryos from the infected callus tissues converted into plantlets tested CSSV negative by PCR/capillary electrophoresis 2 years after weaning. Secondary somatic embryos induced from the CSSV-infected primary somatic embryos revealed the presence of viral fragments at the rate of 4 (4%) and 9 (9%) for T2 and T4, respectively. Real-time PCR revealed 23 of the 24 secondary somatic embryos contained no detectable virus. Based on these findings, it is proposed that progressive elimination of the CSSV in infected cocoa trees occurred from primary embryogenesis to secondary embryogenesis.
AB - Investigations were undertaken on the use of somatic embryogenesis to generate cocoa swollen shoot virus (CSSV) disease free clonal propagules from infected trees. Polymerase chain reaction (PCR) capillary electrophoresis revealed the presence of CSSV in all the callus tissues induced from the CSSV-infected Amelonado cocoa trees (T1, T2 and T4). The virus was transmitted to primary somatic embryos induced from the infected callus tissues at the rate of 10 (19%), 18 (14%) and 16 (15%) for T1, T2 and T4, respectively. Virus free primary somatic embryos from the infected callus tissues converted into plantlets tested CSSV negative by PCR/capillary electrophoresis 2 years after weaning. Secondary somatic embryos induced from the CSSV-infected primary somatic embryos revealed the presence of viral fragments at the rate of 4 (4%) and 9 (9%) for T2 and T4, respectively. Real-time PCR revealed 23 of the 24 secondary somatic embryos contained no detectable virus. Based on these findings, it is proposed that progressive elimination of the CSSV in infected cocoa trees occurred from primary embryogenesis to secondary embryogenesis.
KW - Primary embryogenesis
KW - PCR/capillary electrophoresis
KW - Real-time polymerase chain reaction
KW - Somatic embryos
KW - Secondary embryogenesis
KW - Cocoa swollen shoot virus (CSSV)
U2 - 10.1016/j.jviromet.2008.01.007
DO - 10.1016/j.jviromet.2008.01.007
M3 - Article
C2 - 18294704
VL - 149
SP - 91
EP - 96
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 1
ER -