TY - JOUR
T1 - The expression of Indoleamine 2, 3-dioxygenase (IDO) is reduced in granulomas from BCG vaccinated cattle compared to granulomas from unvaccinated controls after experimental challenge with Mycobacterium bovis
AU - Garcia-Jimenez, Waldo L.
AU - Villarreal-Ramos, Bernardo
AU - Grainger, Duncan
AU - Hewisnon, R. Glyn
AU - Vordermeier, Hans M.
AU - Salguero, Francisco J.
N1 - Crown Copyright © 2018. Published by Elsevier B.V. All rights reserved.
PY - 2018/9/1
Y1 - 2018/9/1
N2 - Bovine tuberculosis (bTB), mainly caused by Mycobacterium bovis (M. bovis), is a major economic disease of livestock worldwide. Vaccination is considered as a potentially sustainable adjunct to the current control strategy. Cattle vaccination with the live attenuated M. bovis bacillus Calmette-Guerin (BCG) confers variable protection; the reasons for this variability are not understood. Indoleamine 2, 3-dioxygenase (IDO), through the catalysis of tryptophan, is thought to have an immunoregulatory role in the immune response to Mycobacterium tuberculosis (M. tuberculosis). In this work, we used immunohistochemistry and digital image analysis to evaluate the presence of IDO in granulomas at different stages of development in cattle that had been BCG-vaccinated or not and then challenged with M. bovis. Our results show that the expression of IDO in granulomas from non-vaccinated M. bovis challenged animals is higher than in granulomas from BCG-vaccinated M. bovis challenged animals. Thus, it is possible that vaccination with BCG prevents the induction of what are thought to be host immunosuppressive pathways by M. bovis, which contribute to pathology during the disease.
AB - Bovine tuberculosis (bTB), mainly caused by Mycobacterium bovis (M. bovis), is a major economic disease of livestock worldwide. Vaccination is considered as a potentially sustainable adjunct to the current control strategy. Cattle vaccination with the live attenuated M. bovis bacillus Calmette-Guerin (BCG) confers variable protection; the reasons for this variability are not understood. Indoleamine 2, 3-dioxygenase (IDO), through the catalysis of tryptophan, is thought to have an immunoregulatory role in the immune response to Mycobacterium tuberculosis (M. tuberculosis). In this work, we used immunohistochemistry and digital image analysis to evaluate the presence of IDO in granulomas at different stages of development in cattle that had been BCG-vaccinated or not and then challenged with M. bovis. Our results show that the expression of IDO in granulomas from non-vaccinated M. bovis challenged animals is higher than in granulomas from BCG-vaccinated M. bovis challenged animals. Thus, it is possible that vaccination with BCG prevents the induction of what are thought to be host immunosuppressive pathways by M. bovis, which contribute to pathology during the disease.
KW - Bovine tuberculosis
KW - IDO
KW - Immunohistochemistry
KW - Indoleamine 2,3-dioxygenase
KW - Mycobacterium bovis
KW - Mycobacterium bovis/immunology
KW - Granuloma/enzymology
KW - Tuberculosis, Bovine/enzymology
KW - Indoleamine-Pyrrole 2,3,-Dioxygenase/metabolism
KW - Lymph Nodes/enzymology
KW - Animals
KW - Cattle
KW - BCG Vaccine/immunology
UR - http://www.scopus.com/inward/record.url?scp=85051780324&partnerID=8YFLogxK
U2 - 10.1016/j.vetimm.2018.08.002
DO - 10.1016/j.vetimm.2018.08.002
M3 - Article
C2 - 30243373
AN - SCOPUS:85051780324
SN - 0165-2427
VL - 203
SP - 52
EP - 56
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
ER -