The maize activator/dissociation system is functional in hexaploid wheat through successive generations

Gabriela M. Pastori, Alison Huttly, Jevon West, Caroline Sparks, Alejandro Pieters, Celina M. Luna, Huw D. Jones, Christine H. Foyer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (SciVal)

Abstract

The aim of the present study was to provide useful background information and evidence of the functionality of the maize Activator/Dissociation (Ac/Ds) system in hexaploid wheat. Two transgenic parental wheat lines, one harbouring the immobilised Ac element (iAc) and the other the Ds element (pUbi[Ds-uidA] bar), were crossed. Transient GUS assays confirmed that the iAc transposase is active in hexaploid wheat. Selected F1 and F2 lines were analysed by PCR using primers specific to Ac, uidA and bar genes. The primer pair Ubi/bar-tag was used to detect excision of the Ds-uidA sequence, which occurred at a frequency of 39% in the F1 generation. Lines free of Ac and showing evidence of Ds excision were subject to Southern analysis, which indicated that at least one transposition event might have occurred in these lines. Although more evidence is required to unequivocally support the reintegration of the Ds element in the wheat genome, the evidence presented here nevertheless demonstrates the effectiveness and potential value of using this system to tag genes in wheat.

Original languageEnglish
Pages (from-to)835-843
Number of pages9
JournalFunctional Plant Biology
Volume34
Issue number9
DOIs
Publication statusPublished - 2007

Keywords

  • RESISTANCE GENE-N
  • TRANSPOSABLE ELEMENT
  • INSERTIONAL MUTAGENESIS
  • TRAPPING SYSTEM
  • RICE PLANTS
  • DS
  • AC
  • FREQUENCY
  • GENOME
  • ARABIDOPSIS

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