Abstract
The permeability parameter (C) for the movement of cephalosporin C across the outer membrane of Pseudomonas aeruginosa was measured using the widely accepted method of Zimmermann & Rosselet. In one experiment, the value of C varied continuously from 4∙2 to 10∙8 cm3 min−1 (mg dry wt cells)−1 over a range of concentrations of the test substrate, cephalosporin C, from 50 to 5 μm. Dependence of C on the concentration of test substrate was still observed when the effect of a possible electric potential difference across the outer membrane was corrected for. In quantitative studies of β-lactam permeation the dependence of C on the concentration of β-lactam should be taken into account.
Original language | English |
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Pages (from-to) | 27-33 |
Number of pages | 7 |
Journal | Microbiology |
Volume | 132 |
Issue number | 1 |
DOIs | |
Publication status | Published - 01 Jan 1986 |