The Permeability Parameter of the Outer Membrane of Pseudomonas aeruginosa Varies with the Concentration of a Test Substrate, Cephalosporin C

R. G. Hewinson, D. C. Lane, M. P. E. Slack, W. W. Nichols

Research output: Contribution to journalArticlepeer-review

Abstract

The permeability parameter (C) for the movement of cephalosporin C across the outer membrane of Pseudomonas aeruginosa was measured using the widely accepted method of Zimmermann & Rosselet. In one experiment, the value of C varied continuously from 4∙2 to 10∙8 cm3 min−1 (mg dry wt cells)−1 over a range of concentrations of the test substrate, cephalosporin C, from 50 to 5 μm. Dependence of C on the concentration of test substrate was still observed when the effect of a possible electric potential difference across the outer membrane was corrected for. In quantitative studies of β-lactam permeation the dependence of C on the concentration of β-lactam should be taken into account.
Original languageEnglish
Pages (from-to)27-33
Number of pages7
JournalMicrobiology
Volume132
Issue number1
DOIs
Publication statusPublished - 01 Jan 1986

Fingerprint

Dive into the research topics of 'The Permeability Parameter of the Outer Membrane of Pseudomonas aeruginosa Varies with the Concentration of a Test Substrate, Cephalosporin C'. Together they form a unique fingerprint.

Cite this