The rapid assignment of ruminal fungi to presumptive genera using ITS1 and ITS2 RNA secondary structures to produce group-specific fingerprints

Danny S. Tuckwell, Matthew J. Nicholson, Christopher S. McSweeney, Michael K. Theodorou, Jayne L. Brookman

Research output: Contribution to journalArticlepeer-review

60 Citations (SciVal)

Abstract

Identification of microbial community members in complex environmental samples is time consuming and repetitive. Here, ribosomal sequences and hidden Markov models are used in a novel approach to rapidly assign fungi to their presumptive genera. The ITS1 and ITS2 fragments from a range of axenic, anaerobic gut fungal cultures, including several type strains, were isolated and the RNA secondary structures predicted for these sequences were used to generate a fingerprinting program. The methodology was then tested and the algorithms improved using a collection of environmentally derived sequences, providing a rapid indicator of the fungal diversity and numbers of novel sequence groups within the environmental sample from which they were derived. While the methodology was developed to assist in investigations involving the rumen ecosystem, it has potential generic application in studying diversity and population dynamics in other microbial ecosystems.
Original languageEnglish
Pages (from-to)1557-1567
Number of pages11
JournalMicrobiology
Volume151
DOIs
Publication statusPublished - 05 May 2005

Keywords

  • ITS, internal transcribed spacer
  • IGER, Institute of Grassland and Environmental Research
  • HMM, hidden Markov model

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