The rapid differentiation of Streptomyces isolates using Fourier transform infrared spectroscopy

Hongjuan J. Zhao, Rachel L. Parry, David Ian Ellis, Gareth W. Griffith, Royston Goodacre

Research output: Contribution to journalArticlepeer-review

32 Citations (SciVal)

Abstract

Fifteen putative Streptomyces spp. isolated from soil were selected to be analysed using Fourier transform infrared (FT-IR) spectroscopy and 16S rRNA gene sequencing. Four colour groupings (groups 1–4) were obtained and described according to the colour of their substrate mycelia, aerial mycelia, spore mass and pigmentation. The dendrogram constructed using unsupervised cluster analysis of the FT-IR data was in good congruence with the four colour groups and the neighbour-joining phylogenetic tree for 16S rDNA sequencing. In particular, those isolates having 100% 16S rDNA similarities which are supposed to be from the same species can be separated from each other using FT-IR analysis. This high throughput method only takes 1–10 s to collect a FT-IR spectrum from each sample, and both 96- and 384-well microplates are available for automated analysis. FT-IR therefore presents itself as a rapid, whole-organism fingerprinting approach which can be used for preliminary differentiation of Streptomyces spp. at sub-species or strain level.
Original languageEnglish
Pages (from-to)213-218
Number of pages6
JournalVibrational Spectroscopy
Volume40
Issue number2
DOIs
Publication statusPublished - 17 Mar 2006

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