The response regulator PhoP4 is required for late developmental events in Myxococcus xanthus

Vinh D Pham, Conrad W Shebelut, Ivy R Jose, David A Hodgson, David E Whitworth, Mitchell Singer

Research output: Contribution to journalArticlepeer-review

7 Citations (SciVal)


Phosphate regulation is complex in the developmental prokaryote Myxococcus xanthus, and requires at least four two-component systems (TCSs). Here, the identification and characterization of a member of one TCS, designated PhoP4, is reported. phoP4 insertion and in-frame deletion strains caused spore viability to be decreased by nearly two orders of magnitude, and reduced all three development-specific phosphatase activities by 80-90 % under phosphate-limiting conditions. Microarray and quantitative PCR analyses demonstrated that PhoP4 is also required for appropriate expression of the predicted pstSCAB-phoU operon of inorganic phosphate assimilation genes. Unlike the case for the other three M. xanthus Pho TCSs, the chromosomal region around phoP4 does not contain a partner histidine kinase gene. Yeast two-hybrid analyses reveal that PhoP4 interacts reciprocally with PhoR2, the histidine kinase of the Pho2 TCS; however, the existence of certain phenotypic differences between phoP4 and phoR2 mutants suggests that PhoP4 interacts with another, as-yet unidentified, histidine kinase.
Original languageEnglish
Pages (from-to)1609-1620
Number of pages12
Issue number6
Publication statusPublished - Jun 2006


  • MI, magnesium-independent
  • QPCR, quantitative PCR
  • TCS, two-component system


Dive into the research topics of 'The response regulator PhoP4 is required for late developmental events in Myxococcus xanthus'. Together they form a unique fingerprint.

Cite this