The RNA helicase, eIF4A-1, is required for ovule development and cell size homeostasis in Arabidopsis

Maxwell S. Bush, Natalie Crowe, Tao Zheng, John Doonan

Research output: Contribution to journalArticlepeer-review

33 Citations (SciVal)
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Abstract

eIF4A is a highly conserved RNA-stimulated ATPase and helicase involved in the initiation of mRNA translation. The Arabidopsis genome encodes 2 isoforms, one of which (eIF4A-1) is required for the coordination between cell cycle progression and cell size. A T-DNA mutant eif4a1 line, with reduced eIF4A protein levels, displays slow growth, reduced lateral root formation, delayed flowering and abnormal ovule development. Loss of eIF4A-1 reduces the proportion of mitotic cells in the root meristem and perturbs the relationship between cell size and cell cycle progression. Several cell cycle reporter proteins, particularly those expressed at G2/M, have reduced expression in eif4a1 mutant meristems. Single eif4a1 mutants are semi-sterile and show aberrant ovule growth, while double eif4a1/eif4a2 homozygous mutants could not be recovered, indicating that eIF4A function is essential for plant growth and development.
Original languageEnglish
Pages (from-to)989-1004
JournalPlant Journal
Volume84
Issue number5
Early online date07 Dec 2015
DOIs
Publication statusPublished - Dec 2015

Keywords

  • cell cycle
  • plant growth
  • translation factor
  • RNA helicase
  • DEAD-box helicase
  • cell size homeostasis
  • Arabidopsis thaliana

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