The role of B cells in the establishment of T cell response in mice infected with an intracellular bacteria, Listeria monocytogenes

Goro Matsuzaki*, H. Martin Vordermeier, Asako Hashimoto, Kikuo Nomoto, Juraj Ivanyi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

41 Citations (SciVal)

Abstract

To clarify the role of B cells in the establishment of T cell response against intracellular bacteria, B-cell-deficient (muMT-/-) mice were infected with an intracellular bacteria, Listeria monocytogenes, and T cell response against the bacteria was analyzed. On day 6 of primary Listeria infection, spleen T cells of the muMT-/- mice showed significantly lower levels of proliferative response and IFN-gamma production than those of normal infected mice after in vitro stimulation with listerial antigen. Even in the secondary Listeria infection after immunization with viable bacteria, spleen T cells of the muMT-/- mice proliferated and produced IFN-gamma against listerial antigen at significantly lower levels than those of normal immunized mice. These results demonstrate participation of B cells in priming of Listeria-specific T cells in vivo. However, B cells failed to present Listeria antigen to Listeria-specific T cells in vitro unless Listeria antigen was solubilized. Furthermore, transfer of immune serum from Listeria-infected normal mice failed to enhance the Listeria-specific T cell response of muMT-/- mice. The results indicate that B cells support the T cell response against intracellular bacteria through a mechanism other than their Ig production or antigen presentation function.

Original languageEnglish
Pages (from-to)178-185
Number of pages8
JournalCellular Immunology
Volume194
Issue number2
DOIs
Publication statusPublished - 15 Jun 1999

Keywords

  • Animals
  • Antibodies, Bacterial/blood
  • Antigen-Presenting Cells/immunology
  • Antigens, CD/biosynthesis
  • B-Lymphocytes/immunology
  • B7-1 Antigen/biosynthesis
  • B7-2 Antigen
  • Female
  • Gene Targeting
  • Immunization, Passive
  • Immunoglobulin mu-Chains/genetics
  • Intracellular Fluid/immunology
  • Listeria monocytogenes/immunology
  • Listeriosis/immunology
  • Male
  • Membrane Glycoproteins/biosynthesis
  • Mice
  • Mice, Inbred C57BL
  • Ovalbumin/immunology
  • Solubility
  • T-Lymphocytes/immunology

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