TY - JOUR
T1 - The Yin-Yang of the Green Fluorescent Protein
T2 - Impact on Saccharomyces cerevisiae stress resistance
AU - Ragon, Mélanie
AU - Bertheau, Lucie
AU - Dumont, Jennifer
AU - Bellanger, Tiffany
AU - Grosselin, Marie
AU - Basu, Mohini
AU - Pourcelot, Eléonore
AU - Horrigue, Walid
AU - Denimal, Emmanuel
AU - Marin, Ambroise
AU - Vaucher, Basile
AU - Berland, Antoine
AU - Lepoivre, Corentin
AU - Dupont, Sébastien
AU - Beney, Laurent
AU - Davey, Hazel
AU - Guyot, Stéphane
N1 - Copyright © 2022 Elsevier B.V. All rights reserved.
PY - 2023/1/1
Y1 - 2023/1/1
N2 - Although fluorescent proteins are widely used as biomarkers (Yin), no study focuses on their influence on the microbial stress response. Here, the Green Fluorescent Protein (GFP) was fused to two proteins of interest in Saccharomyces cerevisiae. Pab1p and Sur7p, respectively involved in stress granules structure and in Can1 membrane domains. These were chosen since questions remain regarding the understanding of the behavior of S. cerevisiae facing different heat kinetics or oxidative stresses. The main results showed that Pab1p-GFP fluorescent mutant displayed a higher resistance than that of the wild type under a heat shock. Moreover, fluorescent mutants exposed to oxidative stresses displayed changes in the cultivability compared to the wild type strain. In silico approaches showed that the presence of the GFP did not influence the structure and so the functionality of the tagged proteins meaning that changes in yeast resistance were certainly related to GFP ROS-scavenging ability (Yang).
AB - Although fluorescent proteins are widely used as biomarkers (Yin), no study focuses on their influence on the microbial stress response. Here, the Green Fluorescent Protein (GFP) was fused to two proteins of interest in Saccharomyces cerevisiae. Pab1p and Sur7p, respectively involved in stress granules structure and in Can1 membrane domains. These were chosen since questions remain regarding the understanding of the behavior of S. cerevisiae facing different heat kinetics or oxidative stresses. The main results showed that Pab1p-GFP fluorescent mutant displayed a higher resistance than that of the wild type under a heat shock. Moreover, fluorescent mutants exposed to oxidative stresses displayed changes in the cultivability compared to the wild type strain. In silico approaches showed that the presence of the GFP did not influence the structure and so the functionality of the tagged proteins meaning that changes in yeast resistance were certainly related to GFP ROS-scavenging ability (Yang).
KW - Green fluorescent protein
KW - Heating kinetics
KW - Membrane patches
KW - Oxidation
KW - Saccharomyces cerevisiae
KW - Stress granules
KW - Yin-Yang
KW - Oxidative Stress/physiology
KW - Saccharomyces cerevisiae/metabolism
KW - Saccharomyces cerevisiae Proteins/genetics
KW - Green Fluorescent Proteins/genetics
UR - http://www.scopus.com/inward/record.url?scp=85144635544&partnerID=8YFLogxK
U2 - 10.1016/j.jphotobiol.2022.112603
DO - 10.1016/j.jphotobiol.2022.112603
M3 - Article
C2 - 36459911
AN - SCOPUS:85144635544
SN - 1011-1344
VL - 238
JO - Journal of Photochemistry and Photobiology B: Biology
JF - Journal of Photochemistry and Photobiology B: Biology
M1 - 112603
ER -