@article{146b7510043543dd9bdeef4dc785d1d7,
title = "The Yin-Yang of the Green Fluorescent Protein: Impact on Saccharomyces cerevisiae stress resistance",
abstract = "Although fluorescent proteins are widely used as biomarkers (Yin), no study focuses on their influence on the microbial stress response. Here, the Green Fluorescent Protein (GFP) was fused to two proteins of interest in Saccharomyces cerevisiae. Pab1p and Sur7p, respectively involved in stress granules structure and in Can1 membrane domains. These were chosen since questions remain regarding the understanding of the behavior of S. cerevisiae facing different heat kinetics or oxidative stresses. The main results showed that Pab1p-GFP fluorescent mutant displayed a higher resistance than that of the wild type under a heat shock. Moreover, fluorescent mutants exposed to oxidative stresses displayed changes in the cultivability compared to the wild type strain. In silico approaches showed that the presence of the GFP did not influence the structure and so the functionality of the tagged proteins meaning that changes in yeast resistance were certainly related to GFP ROS-scavenging ability (Yang).",
keywords = "Green fluorescent protein, Heating kinetics, Membrane patches, Oxidation, Saccharomyces cerevisiae, Stress granules",
author = "M{\'e}lanie Ragon and Lucie Bertheau and Jennifer Dumont and Tiffany Bellanger and Marie Grosselin and Mohini Basu and El{\'e}onore Pourcelot and Walid Horrigue and Emmanuel Denimal and Ambroise Marin and Basile Vaucher and Antoine Berland and Corentin Lepoivre and S{\'e}bastien Dupont and Laurent Beney and Hazel Davey and St{\'e}phane Guyot",
note = "Funding Information: Work carried out at UMR PAM (Dijon, France) was supported by the Regional Council of Bourgogne Franche-Comt{\'e} , the “Fonds Europ{\'e}en de DEveloppement R{\'e}gional (FEDER)”, AgroSup Dijon , I-SITE Bourgogne Franche-Comt{\'e} , Investissement d'Avenir and the international research Master MP 2 “Microbiology and Physicochemistry for Food and Wine Processes” - Universit{\'e} de Bourgogne Franche-Comt{\'e}. Funding Information: Lucie Bertheau and Jennifer Dumont were funded by the Regional Council of Bourgogne Franche-Comt{\'e} and the “Fonds Europ{\'e}en de DEveloppement R{\'e}gional (FEDER)” ( PARI I – AGRALE 2 and PARI II - ALIM+ − 2015-2017 programs). Funding Information: Molecular graphics and analyses performed with UCSF ChimeraX, developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco , with support from National Institutes of Health R01-GM129325 and the Office of Cyber Infrastructure and Computational Biology, National Institute of Allergy and Infectious Diseases . Funding Information: Work carried out at UMR PAM (Dijon, France) was supported by the Regional Council of Bourgogne Franche-Comt{\'e}, the “Fonds Europ{\'e}en de DEveloppement R{\'e}gional (FEDER)”, AgroSup Dijon, I-SITE Bourgogne Franche-Comt{\'e}, Investissement d'Avenir and the international research Master MP2 “Microbiology and Physicochemistry for Food and Wine Processes” - Universit{\'e} de Bourgogne Franche-Comt{\'e}.Lucie Bertheau and Jennifer Dumont were funded by the Regional Council of Bourgogne Franche-Comt{\'e} and the “Fonds Europ{\'e}en de DEveloppement R{\'e}gional (FEDER)” (PARI I – AGRALE 2 and PARI II - ALIM+ − 2015-2017 programs).Fluorescent microscopy. We thank the Plateau Technique d'IMagerie Spectroscopique (PIMS) which is a part of DImaCell Platform (Universit{\'e} de Bourgogne - INRAE, Dijon, France). Plasmid donation. We thank Pr Tatsuya Maeda (University of Tokyo, Japan) who kindly donated the plasmid pTS62 (p415GPD PBP1). RNAseq. We thank members of the Next Generation Sequencing Core Facility (UMS2008 IBSLor, Universit{\'e} de Lorraine-CNRS-INSERM - http://umsibslor.univ-lorraine.fr) for their contribution to this work, Val{\'e}rie Bourguignon-Igel and Dr. Virginie Marchand for RNA extraction and library preparation and Pr Yuri Motorin for bioinformatic analysis of the data. Molecular graphics and analyses performed with UCSF ChimeraX, developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco, with support from National Institutes of Health R01-GM129325 and the Office of Cyber Infrastructure and Computational Biology, National Institute of Allergy and Infectious Diseases. The authors thank Pr Marie ERARD (Institut de Chimie Physique, UMR8000, Universit{\'e} Paris-Saclay, CNRS, 91405 Orsay, FRANCE) for critical reading of the manuscript. Publisher Copyright: {\textcopyright} 2022 Elsevier B.V.",
year = "2023",
month = jan,
day = "1",
doi = "10.1016/j.jphotobiol.2022.112603",
language = "English",
volume = "238",
journal = "Journal of Photochemistry and Photobiology B: Biology",
issn = "1011-1344",
publisher = "Elsevier",
}