The Yin-Yang of the Green Fluorescent Protein: Impact on Saccharomyces cerevisiae stress resistance

Mélanie Ragon, Lucie Bertheau, Jennifer Dumont, Tiffany Bellanger, Marie Grosselin, Mohini Basu, Eléonore Pourcelot, Walid Horrigue, Emmanuel Denimal, Ambroise Marin, Basile Vaucher, Antoine Berland, Corentin Lepoivre, Sébastien Dupont, Laurent Beney, Hazel Davey, Stéphane Guyot*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Abstract

Although fluorescent proteins are widely used as biomarkers (Yin), no study focuses on their influence on the microbial stress response. Here, the Green Fluorescent Protein (GFP) was fused to two proteins of interest in Saccharomyces cerevisiae. Pab1p and Sur7p, respectively involved in stress granules structure and in Can1 membrane domains. These were chosen since questions remain regarding the understanding of the behavior of S. cerevisiae facing different heat kinetics or oxidative stresses. The main results showed that Pab1p-GFP fluorescent mutant displayed a higher resistance than that of the wild type under a heat shock. Moreover, fluorescent mutants exposed to oxidative stresses displayed changes in the cultivability compared to the wild type strain. In silico approaches showed that the presence of the GFP did not influence the structure and so the functionality of the tagged proteins meaning that changes in yeast resistance were certainly related to GFP ROS-scavenging ability (Yang).

Original languageEnglish
Article number112603
Number of pages12
JournalJournal of Photochemistry and Photobiology B: Biology
Volume238
Early online date29 Nov 2022
DOIs
Publication statusPublished - 01 Jan 2023

Keywords

  • Green fluorescent protein
  • Heating kinetics
  • Membrane patches
  • Oxidation
  • Saccharomyces cerevisiae
  • Stress granules
  • Yin-Yang
  • Oxidative Stress/physiology
  • Saccharomyces cerevisiae/metabolism
  • Saccharomyces cerevisiae Proteins/genetics
  • Green Fluorescent Proteins/genetics

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