Tomato 26S Proteasome subunit RPT4a regulates ToLCNDV transcription and activates hypersensitive response in tomato

Pranav Pankaj Sahu, Namisha Sharma, Swati Puranik, Supriya Chakraborty, Manoj Prasad

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)
132 Downloads (Pure)

Abstract

Involvement of 26S proteasomal subunits in plant pathogen-interactions, and the roles of each subunit in independently modulating the activity of many intra- and inter-cellular regulators controlling physiological and defense responses of a plant were well reported. In this regard, we aimed to functionally characterize a Solanum lycopersicum 26S proteasomal subunit RPT4a (SlRPT4) gene, which was differentially expressed after Tomato leaf curl New Delhi virus (ToLCNDV) infection in tolerant cultivar H-88-78-1. Molecular analysis revealed that SlRPT4 protein has an active ATPase activity. SlRPT4 could specifically bind to the stem-loop structure of intergenic region (IR), present in both DNA-A and DNA-B molecule of the bipartite viral genome. Lack of secondary structure in replication-associated gene fragment prevented formation of DNA-protein complex suggesting that binding of SlRPT4 with DNA is secondary structure specific. Interestingly, binding of SlRPT4 to IR inhibited the function of RNA Pol-II and subsequently reduced the bi-directional transcription of ToLCNDV genome. Virus-induced gene silencing of SlRPT4 gene incited conversion of tolerant attributes of cultivar H-88-78-1 into susceptibility. Furthermore, transient overexpression of SlRPT4 resulted in activation of programmed cell death and antioxidant enzymes system. Overall, present study highlights non-proteolytic function of SlRPT4 and their participation in defense pathway against virus infection in tomato.
Original languageEnglish
Article number27078
Number of pages12
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 01 Jun 2016

Keywords

  • Begomovirus/genetics
  • DNA, Viral/chemistry
  • Gene Expression Regulation
  • Genome, Viral
  • Host-Pathogen Interactions/genetics
  • Inverted Repeat Sequences
  • Plant Diseases/genetics
  • Plant Immunity/genetics
  • Plant Proteins/genetics
  • Proteasome Endopeptidase Complex/genetics
  • Protein Binding
  • Protein Subunits/genetics
  • Signal Transduction
  • Solanum lycopersicum/genetics
  • Transcription, Genetic

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