Towards engineered topogenesis of cytochrome b5 and P450 for in vivo transformation of xenobiotics

Naheed N. Kaderbhai, Mustak A. Kaderbhai, Steven Lewis Kelly

Research output: Chapter in Book/Report/Conference proceedingConference Proceeding (Non-Journal item)

Abstract

Nature is endowed with catalysts capable of an unprecedented diversity of biotransformations, beyond the capabilities of synthetic chemistries. In a biotechnological context, there is a growing and emerging need to tap this catalytic potential. CYP (cytochrome P450) represents a superfamily of enzymes capable of a diverse array of catalytic activities. Distinct members are engaged in biosynthetic reactions within many organisms, while others have a role in the detoxification of foreign compounds. The latter substrates include medicines, pollutants, pesticides, carcinogens, perfumes and herbicides, representing considerable applied importance for pharmacology and toxicology. CYPs show a high degree of stereo- and regio-specificity for their reactions, which have wide industrial applications. Recombinant CYPs are commonly expressed as active recombinant cytosolic forms in Escherichia coli. However, selective permeability of E. coli to many substrates and products can cause problems with product recovery when using whole-cell systems. To overcome these problems, we have been developing approaches to facilitate export of functional recombinant haemoproteins to the inner membrane, periplasm and the outer membrane of E. coli. Here, we describe the progress in relation to cytochrome b5 and CYPs.
Original languageEnglish
Title of host publication8th International Symposium on Cytochrome P450 Biodiversity and Biotechnology
Pages1231-1235
Number of pages5
Publication statusPublished - 2006
Event8th International Symposium on Cytochrome P450 Biodiversity and Biotechnology - Swansea, United Kingdom of Great Britain and Northern Ireland
Duration: 23 Jul 200627 Jul 2006

Conference

Conference8th International Symposium on Cytochrome P450 Biodiversity and Biotechnology
Country/TerritoryUnited Kingdom of Great Britain and Northern Ireland
CitySwansea
Period23 Jul 200627 Jul 2006

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