TY - JOUR
T1 - Transcytosis of murine-adapted Bovine Spongiform Encephalopathy agents in an in vitro bovine M cell model
AU - Miyazawa, Kohtaro
AU - Kanaya, Takashi
AU - Takakura, Ikuro
AU - Tanaka, Sachi
AU - Hondo, Tetsuya
AU - Watanabe, Hitoshi
AU - Rose, Michael Terence
AU - Kitazawa, Haruki
AU - Yamaguchi, Takahiro
AU - Katamine, Shigeru
AU - Nishida, Noriyuki
AU - Aso, Hisashi
N1 - Miyazawa, K., Kanaya, T., Takakura, I., Tanaka, S., Hondo, T., Watanabe, H., Rose, M. T., Kitazawa, H., Yamaguchi, T., Katamine, S., Nishida, N., Aso, H. (2010). Transcytosis of murine-adapted Bovine Spongiform Encephalopathy agents in an in vitro bovine M cell model. Journal of Virology, 84, 12285-12291.
Sponsorship: Ministry of Education, Culture, Sports,
Science and Technology, BSE Control Project from the Ministry of
Agriculture, Forestry and Fisheries, and Postdoctoral Fellowships for
Research Abroad from Japan Society for the Promotion of Science
(JSPS).
IMPF: 05.18 RONO: 00
PY - 2010/12
Y1 - 2010/12
N2 - Transmissible spongiform encephalopathies (TSE), including bovine spongiform encephalopathy (BSE), are fatal neurodegenerative disorders in humans and animals. BSE appears to have spread to cattle through the consumption of feed contaminated with BSE/scrapie agents. In the case of an oral infection, the agents have to cross the gut-epithelial barrier. We recently established a bovine intestinal epithelial cell line (BIE cells) that can differentiate into the M cell type in vitro after lymphocytic stimulation (K. Miyazawa, T. Hondo, T. Kanaya, S. Tanaka, I. Takakura, W. Itani, M. T. Rose, H. Kitazawa, T. Yamaguchi, and H. Aso, Histochem. Cell Biol. 133:125-134, 2010). In this study, we evaluated the role of M cells in the intestinal invasion of the murine-adapted BSE (mBSE) agent using our in vitro bovine intestinal epithelial model. We demonstrate here that M cell-differentiated BIE cells are able to transport the mBSE agent without inactivation at least 30-fold more efficiently than undifferentiated BIE cells in our in vitro model. As M cells in the follicle-associated epithelium are known to have a high ability to transport a variety of macromolecules, viruses, and bacteria from gut lumen to mucosal immune cells, our results indicate the possibility that bovine M cells are able to deliver agents of TSE, not just the mBSE agent.
AB - Transmissible spongiform encephalopathies (TSE), including bovine spongiform encephalopathy (BSE), are fatal neurodegenerative disorders in humans and animals. BSE appears to have spread to cattle through the consumption of feed contaminated with BSE/scrapie agents. In the case of an oral infection, the agents have to cross the gut-epithelial barrier. We recently established a bovine intestinal epithelial cell line (BIE cells) that can differentiate into the M cell type in vitro after lymphocytic stimulation (K. Miyazawa, T. Hondo, T. Kanaya, S. Tanaka, I. Takakura, W. Itani, M. T. Rose, H. Kitazawa, T. Yamaguchi, and H. Aso, Histochem. Cell Biol. 133:125-134, 2010). In this study, we evaluated the role of M cells in the intestinal invasion of the murine-adapted BSE (mBSE) agent using our in vitro bovine intestinal epithelial model. We demonstrate here that M cell-differentiated BIE cells are able to transport the mBSE agent without inactivation at least 30-fold more efficiently than undifferentiated BIE cells in our in vitro model. As M cells in the follicle-associated epithelium are known to have a high ability to transport a variety of macromolecules, viruses, and bacteria from gut lumen to mucosal immune cells, our results indicate the possibility that bovine M cells are able to deliver agents of TSE, not just the mBSE agent.
U2 - 10.1128/JVI.00969-10
DO - 10.1128/JVI.00969-10
M3 - Article
C2 - 20861256
SN - 0022-538X
VL - 84
SP - 12285
EP - 12291
JO - Journal of Virology
JF - Journal of Virology
IS - 23
ER -