Transformation of elite wheat varieties for improved end-use qualities: Modification of gibberellin levels and pre-harvest sprouting

H. D. Jones; M. D. Wilkinson

Transformation of elite wheat varieties for improved end-use qualities: Modification of gibberellin levels and pre-harvest sprouting

H. D. Jones, M. D. Wilkinson

Department of Life Sciences

Rothamsted Research

Research output: Chapter in Book/Report/Conference proceeding › Conference Proceeding (Non-Journal item)

Abstract

Wheat was the last of the agriculturally important cereals to be transformed. The ability to introduce genes into wheat remains a technically demanding and relatively inefficient process, mainly confined to culture-responsive but commercially irrelevant genotypes such as Bobwhite. IACR-Rothamsted has developed robust transformation protocols, and in recent years, produced over four hundred independently transformed wheat lines in a wide range of commercial varieties, including Imp, Buster and Rialto. In this paper we summarise our wheat transformation protocols and describe our initial findings from two transformation projects that investigate the role of key proteins in regulating gibberellin biosynthesis and dormancy which have potential to improve end-use quality of bread wheat by reducing pre-harvest sprouting (PHS).

Original language	English
Title of host publication	Wheat in a Global Environment
Subtitle of host publication	Proceedings of the 6th International Wheat Conference, 5-9 June 2000, Budapest, Hungary
Editors	Z. Bedo, L. Láng
Publisher	Springer Nature
Pages	203-209
Number of pages	5
ISBN (Print)	978-0792367222, 0792367227
Publication status	Published - 2001
Event	6th International Wheat Conference - BUDAPEST, Hungary Duration: 05 Jun 2000 → 09 Jun 2000

Publication series

Name	Developments in Plant Breeding
Publisher	Springer
Volume	9

Conference

Conference	6th International Wheat Conference
Country/Territory	Hungary
City	BUDAPEST
Period	05 Jun 2000 → 09 Jun 2000

Keywords

wheat
transformation
preharvest sprouting
gibberellin
dormancy
FERTILE TRANSGENIC WHEAT
MICROPROJECTILE BOMBARDMENT
EXPRESSION
PLANTS
ARABIDOPSIS
TISSUES
GENES

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Jones, H. D., & Wilkinson, M. D. (2001). Transformation of elite wheat varieties for improved end-use qualities: Modification of gibberellin levels and pre-harvest sprouting. In Z. Bedo, & L. Láng (Eds.), Wheat in a Global Environment: Proceedings of the 6th International Wheat Conference, 5-9 June 2000, Budapest, Hungary (pp. 203-209). (Developments in Plant Breeding; Vol. 9). Springer Nature.

Jones, H. D. ; Wilkinson, M. D. / Transformation of elite wheat varieties for improved end-use qualities : Modification of gibberellin levels and pre-harvest sprouting. Wheat in a Global Environment: Proceedings of the 6th International Wheat Conference, 5-9 June 2000, Budapest, Hungary. editor / Z. Bedo ; L. Láng. Springer Nature, 2001. pp. 203-209 (Developments in Plant Breeding).

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abstract = "Wheat was the last of the agriculturally important cereals to be transformed. The ability to introduce genes into wheat remains a technically demanding and relatively inefficient process, mainly confined to culture-responsive but commercially irrelevant genotypes such as Bobwhite. IACR-Rothamsted has developed robust transformation protocols, and in recent years, produced over four hundred independently transformed wheat lines in a wide range of commercial varieties, including Imp, Buster and Rialto. In this paper we summarise our wheat transformation protocols and describe our initial findings from two transformation projects that investigate the role of key proteins in regulating gibberellin biosynthesis and dormancy which have potential to improve end-use quality of bread wheat by reducing pre-harvest sprouting (PHS).",

keywords = "wheat, transformation, preharvest sprouting, gibberellin, dormancy, FERTILE TRANSGENIC WHEAT, MICROPROJECTILE BOMBARDMENT, EXPRESSION, PLANTS, ARABIDOPSIS, TISSUES, GENES",

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Jones, HD & Wilkinson, MD 2001, Transformation of elite wheat varieties for improved end-use qualities: Modification of gibberellin levels and pre-harvest sprouting. in Z Bedo & L Láng (eds), Wheat in a Global Environment: Proceedings of the 6th International Wheat Conference, 5-9 June 2000, Budapest, Hungary. Developments in Plant Breeding, vol. 9, Springer Nature, pp. 203-209, 6th International Wheat Conference, BUDAPEST, Hungary, 05 Jun 2000.

Transformation of elite wheat varieties for improved end-use qualities : Modification of gibberellin levels and pre-harvest sprouting. / Jones, H. D.; Wilkinson, M. D.

Wheat in a Global Environment: Proceedings of the 6th International Wheat Conference, 5-9 June 2000, Budapest, Hungary. ed. / Z. Bedo; L. Láng. Springer Nature, 2001. p. 203-209 (Developments in Plant Breeding; Vol. 9).

Research output: Chapter in Book/Report/Conference proceeding › Conference Proceeding (Non-Journal item)

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AB - Wheat was the last of the agriculturally important cereals to be transformed. The ability to introduce genes into wheat remains a technically demanding and relatively inefficient process, mainly confined to culture-responsive but commercially irrelevant genotypes such as Bobwhite. IACR-Rothamsted has developed robust transformation protocols, and in recent years, produced over four hundred independently transformed wheat lines in a wide range of commercial varieties, including Imp, Buster and Rialto. In this paper we summarise our wheat transformation protocols and describe our initial findings from two transformation projects that investigate the role of key proteins in regulating gibberellin biosynthesis and dormancy which have potential to improve end-use quality of bread wheat by reducing pre-harvest sprouting (PHS).

KW - wheat

KW - transformation

KW - preharvest sprouting

KW - gibberellin

KW - dormancy

KW - FERTILE TRANSGENIC WHEAT

KW - MICROPROJECTILE BOMBARDMENT

KW - EXPRESSION

KW - PLANTS

KW - ARABIDOPSIS

KW - TISSUES

KW - GENES

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Jones HD, Wilkinson MD. Transformation of elite wheat varieties for improved end-use qualities: Modification of gibberellin levels and pre-harvest sprouting. In Bedo Z, Láng L, editors, Wheat in a Global Environment: Proceedings of the 6th International Wheat Conference, 5-9 June 2000, Budapest, Hungary. Springer Nature. 2001. p. 203-209. (Developments in Plant Breeding).

Transformation of elite wheat varieties for improved end-use qualities: Modification of gibberellin levels and pre-harvest sprouting

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