Transformation of oat and inheritance of bar gene expression

B. Kuai, Sophie J. Perret, S. M. Wan, Sue J. Dalton, Andy J. E. Bettany, Phillip Morris

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14 Citations (SciVal)

Abstract

Fertile transgenic plants of oat (Avena sativa L. var. Melys) were produced following microprojectile bombardment of primary embryogenic calli from immature embryos with two plasmids containing the bar gene or the β-glucuronidase (uidA) gene, after selection with glufosinate ammonium. Eleven plants were regenerated from phosphinothricin resistant callus, with three of the eleven plants containing either intact or rearranged copies. No plants co-transformed with the non-selected uidA gene were detected. Stable transmission and expression of the bar gene in the T1 inbred progenies occurred in a Mendelian manner in one line, which contained an intact bar gene, and in all six T2 lines tested from this transformant.
Original languageEnglish
Pages (from-to)79-88
Number of pages10
JournalPlant Cell, Tissue and Organ Culture
Volume66
Issue number2
DOIs
Publication statusPublished - 2001

Keywords

  • Avena sativa
  • Transformation
  • Transgene expression
  • Transgene inheritance

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