Transgenic approaches to study quality traits in cereals

H. D. Jones, H. Wu, C. Sparks, P. R. Shewry

Research output: Chapter in Book/Report/Conference proceedingConference Proceeding (Non-Journal item)

Abstract

Since the first reports of wheat transformation in the early 1990s, protocols continue to be developed using biolistics and Agrobacterium-mediated DNA-delivery into regenerable explants to recover fertile adult transgenic plants. In the majority of cases, the transgene is expressed in a predictable way and segregates during meiosis giving Mendelian ratios of inheritance. Transgenic approaches involving over-expression and targeted gene silencing are increasingly used, both in model and crop species to analyse and validate gene function. We report on developments in the use of Agrobacterium to transform wheat and on transgenic approaches to study the role of HMW glutenin subunits in determining the mixing properties and bread making quality of flour.

Original languageEnglish
Title of host publicationThe BCPC International Congress Crop Science or Technology 2003
Subtitle of host publicationProceedings of the International Congress held in Glasgow, UK, 10-12 November 2003
PublisherBritish Crop Production Council
Pages133-140
Number of pages8
ISBN (Print)978-1901396638, 1901396630
Publication statusPublished - 10 Nov 2003
EventInternational Congress on Crop Science and Technology - Glasgow
Duration: 10 Nov 200312 Nov 2003

Conference

ConferenceInternational Congress on Crop Science and Technology
CityGlasgow
Period10 Nov 200312 Nov 2003

Keywords

  • AGROBACTERIUM-TUMEFACIENS
  • MEDIATED TRANSFORMATION
  • GENETIC-TRANSFORMATION
  • WHEAT
  • EXPRESSION
  • PLANTS
  • VARIETIES
  • PROTEIN
  • SYSTEM

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