Transgenic Wheat, Barley and Oats: Production and Characterization

Paul A Lazzeri, Huw D Jones

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (SciVal)

Abstract

Ever since the first developments in plant transformation technology using model plant species in the early 1980s, there has been a body of plant science research devoted to adapting these techniques to the transformation of crop plants. For some crop species progress was relatively rapid, but in other crop groups such as the small grain cereals, which were not readily amenable to culture in vitro and were not natural hosts to Agrobacterium, it has taken nearly two decades to develop reliable and robust transformation methods.
In the following chapters of this book, transformation procedures for small grain cereals are presented, together with methods for gene and protein expression and the characterization of transgenic plants. In this introductory chapter we try to put these later chapters into context, giving an overview of the development of transformation technology for small grain cereals, discussing some of the pros and cons of the techniques and what limitations still exist.
Original languageEnglish
Title of host publicationTransgenic Wheat, Barley and Oats
Subtitle of host publicationProduction and Characterization Protocols
EditorsHuw D. Jones, Peter R. Shewry
PublisherSpringer Nature
Pages3-20
Number of pages18
Edition1
ISBN (Electronic)978-1597453790
ISBN (Print)978-1588299611
DOIs
Publication statusPublished - 02 Dec 2008

Publication series

NameMethods in Molecular Biology
Volume478
ISSN (Print)1064-3745

Keywords

  • small grain cereals
  • transformation
  • biolistics
  • Agrobacterium
  • tissue culture
  • regeneration
  • selection
  • promoters
  • reporter genes

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