Transient transformation of plants

Huw D Jones, Angela Doherty, Caroline A. Sparks

Research output: Chapter in Book/Report/Conference proceedingChapter

26 Citations (SciVal)

Abstract

Transient expression in plants is a valuable tool for many aspects of functional genomics and promoter testing. It can be used both to over-express and to silence candidate genes. It is also scaleable and provides a viable alternative to microbial fermentation and animal cell culture for the production of recombinant proteins. It does not depend on chromosomal integration of heterologous DNA so is a relatively facile procedure and can lead to high levels of transgene expression. Recombinant DNA can be introduced into plant cells via physical methods, via Agrobacterium or via viral vectors.

Original languageEnglish
Title of host publicationPlant Genomics
EditorsJ. P. Gustafson, Peter Langridge, Daryl J. Somers
PublisherSpringer Nature
Pages131-152
Number of pages22
Volume513
ISBN (Electronic)978-1-59745-427-8
ISBN (Print)978-1-58829-997-0
DOIs
Publication statusPublished - 2009

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume513
ISSN (Print)1064-3745

Keywords

  • Agrobacterium tumefaciens
  • Biolistics
  • DNA, Bacterial
  • DNA, Recombinant
  • Gene Expression
  • Gene Silencing
  • Genetic Techniques
  • Genetic Vectors
  • Plant Viruses
  • Plants, Genetically Modified
  • Plasmids
  • Replicon
  • Transfection
  • Transformation, Genetic
  • Triticum
  • viral-induced gene silencing
  • gene delivery

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