Transplantation of schistosome sporocysts between host snails: A video guide

Gabriel Mouahid, Anne Rognon, Ronaldo de Carvalho Augusto, Patrick Driguez, Kathrin Geyer, Shannon Karinshak, Nelia Luviano, Victoria Mann, Thomas Quack, Kate Rawlinson, George Wendt, Christoph Grunau, Hélène Moné

Research output: Contribution to journalArticlepeer-review

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Abstract

Schistosomiasis is an important parasitic disease, touching roughly 200 million people worldwide. The causative agents are different Schistosoma species. Schistosomes have a complex life cycle, with a freshwater snail as intermediate host. After infection, sporocysts develop inside the snail host and give rise to human dwelling larvae. We present here a detailed step-by-step video instruction in English, French, Spanish and Portuguese that shows how these sporocysts can be manipulated and transferred from one snail to another. This procedure provides a technical basis for different types of ex vivo modifications, such as those used in functional genomics studies
Original languageEnglish
JournalWellcome Open Research
Early online date09 Jan 2018
DOIs
Publication statusPublished - 09 Jan 2018

Keywords

  • schistosomiasis
  • sporocyst transfer
  • biomphalaria
  • video instruction

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