Use of a colon simulation technique to assess the effect of live yeast on fermentation parameters and microbiota of the colon of pig

E. Pinloche, M. Williams, R. D'Inca, E. Auclair, C. J. Newbold

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Abstract

The impact of 2 doses of a Saccharomyces cerevisiae were evaluated, 5 × 10(10) cfu/kg of feed (L1) and 5 × 10(11) cfu/kg of feed (L2) against a control (CON) with no added yeast, using an in vitro model [colon simulation technique (Cositec)] to mimic digestion in the pig colon. The L2 (but not L1) dose significantly improved DM digestibility compared to CON (61 v 58%) and increased NH(3) concentrations (+15%). Volatile fatty acid concentrations increased with L2 compared to CON--isobutyrate (+13.5%), propionate (+8.5%), isovalerate (+17.8%), and valerate (+25%)--but only valerate was increased with L1 (+14.2%). The analysis of microbiota from the liquid associated bacteria (LAB) and solid associated bacteria (SAB) revealed an interaction between the fraction and treatment (P <0.05). Indeed, L2 had a significant impact on SAB and LAB (P <0.01) whereas L1 only tended to change the structure of the population in the SAB (P <0.1). Overall, this study showed that a live yeast probiotic could improve digestion in a colonic simulation model but only at the higher dose used and this effect was associated with a shift in the bacterial population therein.
Original languageEnglish
Pages (from-to)353-355
Number of pages3
JournalJournal of Animal Science
Volume90
Issue numberSupplement 4
DOIs
Publication statusPublished - Dec 2012

Keywords

  • Animals
  • Colon
  • Fermentation
  • Hydrogen-Ion Concentration
  • Models, Biological
  • Oxidation-Reduction
  • Saccharomyces cerevisiae
  • Swine

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