Using next-generation sequencing to determine diversity of horse intestinal worms: Identifying the equine ʼnemabiome'

Catriona Mitchell, Catherine O'Sullivan, Eric Jean-Yves Roger Pinloche, Toby Wilkinson, Russ Morphew, Neil McEwan

Research output: Contribution to journalArticlepeer-review

16 Citations (SciVal)
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Abstract

Next-generation sequencing of DNA from nematode eggs has been utilised to give the first account of the equine ʼnemabiome'. In all equine faecal samples investigated, multiple species of Strongylidae were detected, ranging from 7.5 (SEM 0.79) with 99+% identity to sequences in the NCBI database to 13.3 (SEM 0.80) with 90+% identity. This range is typical of the number of species described previously in morphological studies using large quantities of digesta per animal. However, the current method is non-invasive; relies on DNA analysis, avoiding the need for specialist microscopy identification; and can be carried out with small samples, providing significant advantages over current methods.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalJournal of Equine Science
Volume30
Issue number1
DOIs
Publication statusPublished - Mar 2019

Keywords

  • Diversity
  • Faecal egg
  • Horse
  • Nemabiome

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