Projects per year
Abstract
Understanding rumen plant-microbe interactions is central for development of novel methodologies allowing improvements in ruminant nutrient use efficiency. This study investigated rumen bacterial colonisation of fresh plant material and changes in plant chemistry over a period of 24 h period using three different fresh forages: Lolium perenne (perennial ryegrass; PRG), Lotus corniculatus (birds foot trefoil; BFT) and Trifolium pratense (red clover; RC). We show using 16S rDNA ion torrent sequencing that plant epiphytic populations present pre-incubation (0 h) were substantially different to those attached post incubations in the presence of rumen fluid on all forages. Thereafter primary and secondary colonisation events were evident as defined by changes in abundances of attached bacteria and changes in plant chemistry, as assessed using Fourier Tansfrom-Infra Red (FT-IR) spectroscopy. For PRG colonisation, primary colonisation was seen up to 4 h and secondary colonisation from 4 h onwards. These changes from primary to secondary colonisation occurred significantly later with BFT and RC, with primary colonisation being up to 6 h and secondary colonisation post 6 h of incubation. Across all 3 forages the main colonising bacteria present at all time points post-incubation were Prevotella, Pseudobutyrivibrio, Ruminococcus, Olsenella, Butyrivibrio and Anaeroplasma (14.2, 5.4, 1.9, 2.7, 1.8, 2.0% on average respectively) with Pseudobutyrivibrio and Anaeroplasma having a higher abundance during secondary colonisation. Using CowPI we predict that there were differences between bacterial metabolic function during primary and secondary colonisation. Specifically, carbohydrate metabolism were predicted to be increased in the bacteria attached during secondary colonisation, irrespective of forage type. The CowPI data coupled with the FT-IR plant chemistry data suggest that attached bacterial function is similar irrespective of forage type, with the main changes occurring between primary and secondary colonisation. These data suggest that the sward composition of pasture may have major implications for the temporal availability of nutrients for animal.
Original language | English |
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Article number | 2184 |
Journal | Frontiers in Microbiology |
Volume | 9 |
DOIs | |
Publication status | Published - 19 Sept 2018 |
Keywords
- microbiome
- perennial ryegrass
- birds foot trefoil
- red clover
- 16S rRNA gene
- FTIR
- CowPI
- rumen
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Dive into the research topics of 'Using 'Omic Approaches to Compare Temporal Bacterial Colonization of Lolium perenne, Lotus corniculatus and Trifolium pratense in the Rumen'. Together they form a unique fingerprint.Profiles
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Alison Kingston-Smith
- Faculty of Earth and Life Sciences, Institute of Biological, Environmental & Rural Sciences (IBERS) - Chair
Person: Teaching And Research
Projects
- 3 Finished
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Future Forages: Implications of forage response to climate change for ruminant production
Kingston-Smith, A., Creevey, C. & Hart, E.
Biotechnology and Biological Sciences Research Council
02 Jul 2018 → 31 Dec 2021
Project: Externally funded research
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Characterisation and exploitation of novel antimicrobials within the rumen microbiota
Huws, S., Creevey, C., Kingston-Smith, A., McEwan, N. & Newbold, C.
Biotechnology and Biological Sciences Research Council
01 Apr 2014 → 31 Mar 2018
Project: Externally funded research
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RSB : Rumen Systems Biology
Biotechnology and Biological Sciences Research Council
01 Apr 2012 → 31 Mar 2017
Project: Externally funded research