TY - JOUR
T1 - Utility of multidisciplinary approach for genome diagnostics of cultivated and wild germplasm resources of medicinal Withania somnifera, and status of new species, W. ashwagandha, to the cultivated taxon
AU - Dar, Tanvir H.
AU - Sehgal, Deepmala
AU - Koul, Sushma
AU - Mir, Bilal A.
AU - Kaul, Maharaj K.
AU - Raina, Soom Nath
AU - Qazi, Ghulam N.
AU - Kumar, Arun
N1 - Kumar, A., Mir, B.A., Sehgal, D., Dar, T.H., Koul, S., Kaul, M. K., Raina, S. N., Qazi, G. N. (2010). Utility of multidisciplinary approach for genome diagnostics of cultivated and wild germplasm resources of medicinal Withania somnifera, and status of new species, W. ashwagandha, to the cultivated taxon. Plant Systematics and Evolution, 291, (3-4), 141-151.
IMPF: 01.36 RONO: 00
PY - 2010
Y1 - 2010
N2 - Realizing the inconsistencies that exist in the
extent and nature of differentiation in the Withania somnifera
genetic resources in India, the 21 cultivated and wild
accessions, and the two hybrids (cultivated 9 wild accessions
and vice versa) were investigated for morphological,
cytogenetical, chemical profiling, and crossability features.
Their nuclear and chloroplast genomes were also assayed
at the nucleotide sequence level, and by use of DNA
markers. Chloroplast DNA diversity and somatic chromosome
number (2n = 48) were not helpful in identifying the
differences. Other approaches, on the other hand, especially
restriction endonuclease digests, types and sequence
length composition of ITS 1 and ITS 2 of nuclear ribosomal
DNA, AFLP fingerprinting, and crossability barriers
unambiguously provided startling discrete differences
between the cultivated and wild accessions, indicating a
clear division of W. somnifera into two distinct lineages.
These data, therefore, are indicative of the fact that because
of the unique characteristics of its nuclear genome, and
strong crossability barriers vis-a`-vis wild accessions of
W. somnifera, the cultivated accessions should be relegated
to the rank of the separate species, W. ashwagandha.
AB - Realizing the inconsistencies that exist in the
extent and nature of differentiation in the Withania somnifera
genetic resources in India, the 21 cultivated and wild
accessions, and the two hybrids (cultivated 9 wild accessions
and vice versa) were investigated for morphological,
cytogenetical, chemical profiling, and crossability features.
Their nuclear and chloroplast genomes were also assayed
at the nucleotide sequence level, and by use of DNA
markers. Chloroplast DNA diversity and somatic chromosome
number (2n = 48) were not helpful in identifying the
differences. Other approaches, on the other hand, especially
restriction endonuclease digests, types and sequence
length composition of ITS 1 and ITS 2 of nuclear ribosomal
DNA, AFLP fingerprinting, and crossability barriers
unambiguously provided startling discrete differences
between the cultivated and wild accessions, indicating a
clear division of W. somnifera into two distinct lineages.
These data, therefore, are indicative of the fact that because
of the unique characteristics of its nuclear genome, and
strong crossability barriers vis-a`-vis wild accessions of
W. somnifera, the cultivated accessions should be relegated
to the rank of the separate species, W. ashwagandha.
U2 - 10.1007/s00606-010-0372-4
DO - 10.1007/s00606-010-0372-4
M3 - Article
SN - 1615-6110
VL - 291
SP - 141
EP - 151
JO - Plant Systematics and Evolution
JF - Plant Systematics and Evolution
IS - 3-4
ER -