Xylanolytic activity of the rumen protozoan Diploplastron affine

K. Wereszka, T. Michalowski, C. J. Newbold, N. R. McEwan

Research output: Contribution to journalArticlepeer-review


Cell free extract prepared from ciliates Diploplastron affine free from bacteria degraded xylan releasing reducing sugars at the rate of 117.8 +/- 3.14 mu M/mg protein/h. Two xylan degrading enzymes have been identified in protozoal cell free extract by a zymogram technique it was also found that one of the identified enzymes degraded xylan to xylooligosaccharides i.e. was of endoxylanase character. Screening of the Diploplastron affine cDNA library resulted in the identification of a xylamse encoding gene consisting of 1670 bp.

Original languageEnglish
Pages (from-to)43-46
Number of pages4
JournalJournal of Animal and Feed Sciences
Issue numberSupplement 1
Publication statusPublished - 2006


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