Yeast-surface expressed BVDV E2 protein induces a Th1/Th2 response in naïve T cells

Robert Patterson, Jessica Nerren, Michael Kogut, Pinar Court, Bernardo Villarreal-Ramos, Hans Martin Seyfert, Paul Dalby, Dirk Werling

Research output: Contribution to journalArticlepeer-review

Abstract

Yeast species such as Saccharomyces cerevisiae are known to be potent activators of the immune system. S. cerevisiae activates the innate immune system by engaging pattern recognition receptors such as toll like receptor 2 (TLR2) and dectin-1. In the current project, we express the immunogenic envelope protein E2 of bovine viral diarrhoea virus (BVDV) on the surface of S. cerevisiae. After successful expression, components of the innate and adaptive immune response induced by the recombinant S. cerevisiae in vitro were analysed to determine if expression in yeast enhances the immunogenicity of the viral protein. Recombinant S. cerevisiae stimulated production of the chemokine CXCL-8 in primary bovine macrophages, but did no stimulate production of reactive oxygen species (ROS) in the same cells. Additionally, bovine macrophages primed with S. cerevisiae expressing viral envelope proteins had a greater capacity for stimulating proliferation of CD4+ T-cells from BVDV-free animals compared to macrophages primed with envelope protein alone or S. cerevisiae without envelope protein expression. Heat inactivation of recombinant S. cerevisiae increased ROS production and capacity to stimulate CD4+ T-cells in macrophages but did not alter CXCL-8 release compared to the live counter-part. Additionally, heat-inactivation of recombinant S. cerevisiae induced less INFγ and IL-4 but equal amounts of IL-10 compared to live yeast T-cell cultures. Our studies demonstrate a use for S. cerevisiae as a vehicle for transporting BVDV vaccine antigen to antigen-presenting cell in order to elicit cell-mediated immunity even in naïve animals.

Original languageEnglish
Pages (from-to)107-114
Number of pages8
JournalDevelopmental and Comparative Immunology
Volume37
Issue number1
Early online date31 Oct 2011
DOIs
Publication statusPublished - 01 May 2012

Keywords

  • BVD
  • Cattle
  • Dectin-1
  • E2
  • Saccharomyces cerevisiae
  • TLR2

Fingerprint

Dive into the research topics of 'Yeast-surface expressed BVDV E2 protein induces a Th1/Th2 response in naïve T cells'. Together they form a unique fingerprint.

Cite this